- Title
- Fat1 cadherin provides a novel minimal residual disease marker in acute lymphoblastic leukemia
- Creator
- Ardjmand, Alireza; de Bock, Charles E.; Shahrokhi, Somayeh; Lincz, Lisa F.; Boyd, Andrew W.; Burns, Gordon F.; Thorne, Rick F.
- Relation
- NHMRC.455531
- Relation
- Hematology Vol. 18, Issue 6, p. 315-321
- Publisher Link
- http://dx.doi.org/10.1179/1607845413Y.0000000080
- Publisher
- Maney Publishing
- Resource Type
- journal article
- Date
- 2013
- Description
- Measurement of minimal residual disease (MRD) maintains an important role in the clinical management of acute lymphoblastic leukemia (ALL). Recently, we identified Fat1 cadherin as a unique and independent prognostic factor for relapse-free and overall survival in pediatric pre-B-ALL. Here, we analyzed Fat1 mRNA for its potential as a novel marker of MRD in cases of pre-B- and T-ALL. Analyses of microarray data from 125 matched diagnosis/relapse samples across three independent datasets indicate that Fat1 mRNA is detectable in an average of 31.3% of diagnosed pre-B-ALL, of which 67.5% of cases remained positive at relapse. Furthermore, some 20% of cases with undetectable levels of Fat1 mRNA at diagnosis became positive upon relapse. T-ALL cases were 83.3% positive for Fat1 expression at diagnosis with 77.7% remaining positive at relapse. Towards proof of concept, we developed a quantitative polymerase chain reaction assay and demonstrate detection of Fat1 mRNA in leukemic cells mixed with normal peripheral blood cells at a sensitivity of 1 in 10 000 to 100 000 cells. Fat1 may therefore provide a new marker of MRD for patients with ALL lacking known genomic aberrations or within a multiplex approach to MRD detection.
- Subject
- acute lymphoblastic leukemia; fat cadherins; minimal residual disease; pre-B-ALL; T-ALL
- Identifier
- http://hdl.handle.net/1959.13/1295183
- Identifier
- uon:18969
- Identifier
- ISSN:1024-5332
- Language
- eng
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